Labeled Nucleoside Triphosphates

Nucleoside Triphosphates | Labeled Nucleoside Triphosphates

There are many applications for Labeled Nucleoside Triphosphates. The following excerpt from Dr. Rosenblum’s article (New dye-labeled terminators for improved DNA sequencing patterns, B. B. Rosenblum, L. G. Lee, S. L. Spurgeon, S. H. Khan, S. M. Menchen, C. R. Heiner, and S. M. Chen; Nucleic Acids Research, Volume 25, Issue 22, p. 4500-4504) describes it very well:

“Automated DNA sequencing uses fluorescent dyes for the detection of the electrophoretically resolved DNA fragments. Two variations of automated DNA sequencing have evolved: dye-labeled primer sequencing (2–4), in which the fluorescent dyes are attached to the 5' end of the primer oligonucleotide, and dye-labeled terminator sequencing, in which the dyes are attached to the terminating dideoxynucleoside triphosphates (5,6). Each sequencing method has advantages and disadvantages.

Dye-labeled primer sequencing has benefited from the development of DNA polymerases which do not discriminate between deoxy- and dideoxynucleotides (7,8). These polymerases provide sequencing electropherograms with very even peak heights. Base-calling is easy and reliable, and the ability to call heterozygotes can be based on peak heights as well as the presence of two bases at a position (9,10). The major disadvantage of the dye primer method is the requirement for four separate extension reactions and four dye-labeled primers for each template.

The major advantages of dye-labeled terminator sequencing are convenience, since only a single extension reaction is required for each template, and the synthesis of a labeled primer is unnecessary, allowing the use of preferred hybridization sites. In addition false terminations, in which the DNA fragments are terminated by a deoxynucleotide rather than a dideoxynucleotide, are not observed as these products are unlabeled. The major disadvantage of dye-labeled terminators is that with every polymerase the pattern of termination with dye-labeled terminators has been found to be less even than for dye-labeled primers. The presence of very small or very large peaks can result in errors in automated base-calling.”

Product Family
Labeled 2’-Deoxyribonucleoside Triphosphates
Labeled 2’,3’-Dideoxyribonucleoside Triphosphates
Labeled Ribonucleoside Triphosphates

Labeled Nucleoside Triphosphates

Nucleoside Triphosphates currently in inventory and available for immediate shipment:

  • Non-Modified Nucleoside Triphosphates
  • Base Modified Nucleoside Triphosphates
      AA-dCTP, AA-dUTP, AA-CTP, AA-UTP, 8-Amino-ATP
  • Phosphate Modified Nucleoside Triphosphates
      Alpha-thio-dATP, Alpha-thio-dCTP, Alpha-thio-dGTP, Alpha-thio-TTP, Alpha-thio-ATP, Alpha-thio-CTP, Alpha-thio-GTP, Alpha-thio-UTP
  • Sugar Modified Nucleoside Triphosphates
      2’-Fluoro-dCTP, 2’-Fluoro-dUTP, ddATP, ddCTP, ddGTP, dTTP, ddUTP, 2'-O-Me-ATP, 2'-O-Me-CTP, 2'-O-Me-GTP, 2'-O-Me-UTP
  • Phosphate and Sugar Modified Nucleoside Triphosphates
      Alpha-thio-ddATP, Alpha-thio-ddCTP, Alpha-thio-ddGTP, Alpha-thio-dTTP
  • Biotin Nucleotide Analogs
      Biotin-5-dCTP, Biotin-11-dCTP, Biotin-16-dCTP, Biotin-5-dUTP, Biotin-11-dUTP, Biotin-16-dUTP, Biotin-5-CTP, Biotin-11-CTP, Biotin-16-CTP, Biotin-5-UTP, Biotin-11-UTP, Biotin-16-UTP
  • Cyanine Dye Nucleotide Analogs
      SulfoCyanine 3-dCTP, SulfoCyanine 3-dUTP, SulfoCyanine 5-dCTP, SulfoCyanine 5-dUTP, SulfoCyanine 3-CTP, SulfoCyanine 3-UTP, SulfoCyanine-5-CTP, SulfoCyanine-5-UTP
  • Antiviral Nucleotides
      3TCMP, 3TCTP, AzTMP, AzTTP, d4TMP, d4TTP, Ribavirin-5‘-triphosphate
  • Labeled Nucleoside Triphosphates
      Dye-Labeled-5-Aminoallyl-dUTP, Biotin Labeled-5-Aminoallyl-dUTP, Dye-Labeled-5-Aminoallyl-dCTP, Biotin Labeled-5-Aminoallyl-dCTP, Dye-Labeled-5-Aminoallyl-ddUTP, Biotin Labeled-5-Aminoallyl-ddUTP, Dye-Labeled-5-Aminoallyl-ddCTP, Biotin Labeled-5-Aminoallyl-ddCTP, Dye-Labeled-5-Aminoallyl-UTP, Biotin Labeled-5-Aminoallyl-UTP, Dye-Labeled-5-Aminoallyl-CTP, Biotin Labeled-5-Aminoallyl-CTP
  • Click Chemistry
      C8-A-dUTP, C8-A-dCTP, 5-E-UTP, 5-E-dUTP, EdU-488, EdU-555, EdU-594, EdU-647, EdU-FC-488, EdU-FC-555, EdU-FC-594, EdU- FC-647, EdU HTS Kit 488, EdU HTS Kit 555, EdU HTS Kit 594, EdU Click 488 Kit, EdU Click 555 Kit, EdU Click 594 Kit, EdU Click 647 Kit, EdU Flow Cytometry488, EdU Flow Cytometry555, EdU Flow Cytometry594, EdU Flow Cytometry647, EdU HTS488- 2x96 Kit, EdU HTS555- 2x96 Kit, EdU HTS594- 2x96 Kit, EdU HTS647- 2x96 Kit

Nucleoside Triphosphates in alphabetical order:

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