Labeled Nucleoside Triphosphates

Nucleoside Triphosphates | Labeled Nucleoside Triphosphates

There are many applications for Labeled Nucleoside Triphosphates. The following excerpt from Dr. Rosenblum’s article (New dye-labeled terminators for improved DNA sequencing patterns, B. B. Rosenblum, L. G. Lee, S. L. Spurgeon, S. H. Khan, S. M. Menchen, C. R. Heiner, and S. M. Chen; Nucleic Acids Research, Volume 25, Issue 22, p. 4500-4504) describes it very well:

“Automated DNA sequencing uses fluorescent dyes for the detection of the electrophoretically resolved DNA fragments. Two variations of automated DNA sequencing have evolved: dye-labeled primer sequencing (2–4), in which the fluorescent dyes are attached to the 5' end of the primer oligonucleotide, and dye-labeled terminator sequencing, in which the dyes are attached to the terminating dideoxynucleoside triphosphates (5,6). Each sequencing method has advantages and disadvantages.

Dye-labeled primer sequencing has benefited from the development of DNA polymerases which do not discriminate between deoxy- and dideoxynucleotides (7,8). These polymerases provide sequencing electropherograms with very even peak heights. Base-calling is easy and reliable, and the ability to call heterozygotes can be based on peak heights as well as the presence of two bases at a position (9,10). The major disadvantage of the dye primer method is the requirement for four separate extension reactions and four dye-labeled primers for each template.

The major advantages of dye-labeled terminator sequencing are convenience, since only a single extension reaction is required for each template, and the synthesis of a labeled primer is unnecessary, allowing the use of preferred hybridization sites. In addition false terminations, in which the DNA fragments are terminated by a deoxynucleotide rather than a dideoxynucleotide, are not observed as these products are unlabeled. The major disadvantage of dye-labeled terminators is that with every polymerase the pattern of termination with dye-labeled terminators has been found to be less even than for dye-labeled primers. The presence of very small or very large peaks can result in errors in automated base-calling.”

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Labeled Nucleoside Triphosphates

Nucleoside Triphosphates currently in inventory and available for immediate shipment:

Nucleoside Triphosphates in alphabetical order:

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